Figure 5.

Additional dosing of VGX-3100 increases lytic protein content of activated (CD137+) CD8+ T cells. (a) Representative staining of the activation marker CD137 (also known as 4-1BB) as well as Granzyme B and Perforin in CD8+ T cells. (b) Antigen-specific expression of CD137 on patient CD8+ T cells in response to extended stimulation with VGX-3100 antigens prior to and following the boost (left panel). The right panel displays data regarding cosynthesis and expression of Granzyme B and Perforin within the activated (CD137+) CD8+ T cell subset in response to VGX-3100 antigens (Human Papillomavirus (HPV) peptide stimulation). Irr Pep = Irrelevant Peptide. HPV Pep = peptides spanning HPV16 and HPV18 E6 and E7