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Dose-dependent effect of 3O-C12-HSL on MUC5AC synthesis. Cells were stimulated with 1, 10, or 100 μM 3O-C12-HSL. (A) The mRNA level of MUC5AC expression at 8 h after the addition of 3O-C12-HSL was analyzed by RT-PCR. (B) MUC5AC protein was measured by an ELISA at 12 h after the addition of 3O-C12-HSL. Data are expressed as the mean and SEM for three experiments. An asterisk and a dagger indicate P values of <0.05 and <0.01, respectively, for comparisons with culture medium alone.
