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Phosphorylation of I-κB and ERK1/2 induced by 3O-C12-HSL. Cells were treated with 3O-C12-HSL for 10 min and evaluated by Western blotting. (A) I-κB phosphorylation was induced in 3O-C12-HSL-treated cells but not in untreated cells. (B) ERK1/2 phosphorylation was induced in 3O-C12-HSL-treated cells. This phosphorylation was blocked by the addition of azithromycin. Data are representative of three separate experiments. p, phospho.
