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Effect of PD98059 on MUC5AC production in cells activated by 3O-C12-HSL. Cells were pretreated with 50 μM PD98059 for 30 min prior to 3O-C12-HSL stimulation. (A) MUC5AC mRNA expression was determined by RT-PCR. (B) MUC5AC protein was measured by an ELISA. Data are expressed as the mean and SEM for three experiments. An asterisk indicates a P value of <0.05 for a comparison with culture medium alone.
