Table 1. Primer sequences used for real-time quantitative PCR (QPCR).
Transcript | Direction | Primer Sequence (5' to 3') |
---|---|---|
Mutant Tnnt2 | Forward | CGAGCAGCAGCGTATTTGG |
Reverse | CAGCCTTCCTCCTGTTCTCCTC | |
Wildtype Tnnt2 | Forward | CGAGCAGCAGCGTATTCGC |
Reverse | CAGCCTTCCTCCTGTTCTCCTC | |
Total Tnnt2 | Forward | GGGCCGAGCAGCAGCGTATT |
Reverse | CAGCCTTCCTCCTGTTCTCCTC |