Fig 2.

 Dot blot analysis of Bombyx mori protein disulfide isomerase (bPDI) messenger ribonucleic acid (RNA) expression in tissues. Total RNA was isolated from the following organs: S, silk gland; F, fat body; M, midgut; Ma, Malpighian tubules; E, epidermis. Total RNA (5 μg) from each sample was transferred onto a Hybond-N nylon membrane. Hybridization was carried out using [α-³²P]deoxyadenosine triphosphate–labeled bPDI complementary deoxyribonucleic acid as a probe. The bPDI band intensities were measured using ImageQuant software. The bar graph shows the averages of the quantified data from 3 independent experiments, and the inset shows the results from 1 representative experiment. 18S ribosomal RNA was used as a quantitation marker