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. 2016 Dec 9;11(12):e0167789. doi: 10.1371/journal.pone.0167789

Fig 4. Ultrastructural localization of EDMTFH by immunogold labeling.

Fig 4

Downfeathers and scales of chicken embroys at days 16 (16d) and 18 (18d) of development were labeled for EDMTFH either without (A-C, G) and with (D-F) silver enhancement. (A) Diffuse labeling over corneous bundles (arrow) of barbule cells (bl). (B) Diffuse labeling in the corneous bundles (arrow) of a subperiderm cell in a scale. (C) Immuno-negative control section of a barbule. (D) Labeling cytoplasmic corneous bundles (arrowheads) but not the cytoplasm (cy) in a barbule cell (bl). (E) Close-up to show the association of the labeling with corneous bundles (arrows). (F) Early differentiating barbule cell with short corneous bundles (arrows). (G) Double-labeling for EDMTFH (5 nm gold particles) and feather beta-keratin (20 nm gold particles) in a barbule cell. Note that the large particles appear to be more abundant than the small particles. A lower magnification image of the double-labeling is shown in S3 Fig. Bars: 100 nm (A, B); 200 nm (C-F); 50 nm (G).