Fig 1. Evaluation of flow cytometry gating strategy for MVs in plasma.

Suspensions of neutrophil-derived MVs and MV-depleted healthy volunteer plasma were stained with antibodies, individually or after addition of MVs to plasma (volume ratio: 1:4). Larger events were excluded using 1.3 μm beads (not shown) and a gate (R1) for neutrophil-derived MVs was drawn based on CD66b+, CD11b+ double-staining and a cut-off boundary defined by detergent-mediated selective lysis of MVs. Isotype-matched control staining provided confirmation of the detergent-lysis method for identification of non-MV background events.