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Expression of TmPLB1 in P. pastoris GS115. (A) SDS-PAGE analysis of TmPLB1 expressed in P. pastoris GS115 fermentation supernatants in shaking flask cultures. Lanes 1–8, P. pastoris induced with methanol for 6, 12, 24, 48, 72, 96, 120 and 144 h; M, standard protein marker (Fermentas, Burlington, ON, Canada). (B) Western blot analysis of TmPLB1. Lane 1, empty vector control; lane 2, TmPLB1 expression plasmids.
