Fig 2.

 Luciferase (LUC) heat denatured in the presence of recombinant Hsp30C (30C) can be reactivated in vitro by reticulocyte lysate (RRL). LUC (0.2 μM) was incubated with 6.0 μM of 30C (○) or bovine serum albumin (x), and heat denatured at 42°C or kept at 22°C (♦) for 15 minutes. Samples were adjusted to 60 % RRL and 6 μM adenosine triphosphate (ATP), and incubated at 30°C for various periods of time. LUC activity was assayed as described in “Materials and Methods.” Some samples were heat treated without 30C (▪) or assayed without ATP (▴). The data are calculated as a percentage of the activity of unheated LUC at 0 minute, and expressed as the mean of 4–6 trials ± SE