Fig 3.

 The effect of different amounts of recombinant Hsp30C (30C) during heat denaturation on luciferase (LUC) enzyme reactivation in vitro. LUC (0.2 μM) was heat denatured at 42°C with various molar quantities at 30C-LUC molar ratios, as indicated beside each curve. LUC activity was assayed after incubation with reticulocyte lysate and adenosine triphosphate, as described in “Materials and Methods.” All data are expressed as a percentage of the activity of native LUC and shown as the mean ± SE