Fig 9.

 Luciferase (LUC) can be refolded in vitro when chemically denatured in the presence of recombinant Hsp30C (30C) and N-30C, but not C-30C. LUC (0.05 μM) was maintained in refolding buffer (♦) or incubated in 0.5 M guanidine hydrochloride in the absence (•) or presence of either 1.5 μM 30C (□) or N-30C (▴) or C-30C (▵) for 1 hour at 22°C. The samples were then assayed in the reticulocyte lysate refolding assay as described in “Materials and Methods.” In some samples 30C was added after the chemical treatment and prior to the refolding assay (X). Data are expressed as a percentage of native LUC activity and expressed as the mean of 3 trials ± SE