Fig 3. Matriptase can undergo autoactivation in cells that do not express prostasin.

Human hematological cancer cell lines Raji, Namalwa, and Ramos, human prostate cancer cell lines DU145 and PC3, and human hepatoma Hep3B cells were treated with PBS as the non-activation control (lanes 1) or pH 6.0 buffer for 20 min to induce matriptase activation. Cell lysates were prepared and analyzed by Western blot analysis for matriptase species using the matriptase specific mAb M24. The matriptase species observed include matriptase zymogen (band a), matriptase-HAI-2 complex (band b), and matriptase-HAI-1 complex (band c). This study was conducted multiple times (n>5) with the same profile observed. Representative data are shown.