Fig 1.

 Expression of U14 small nucleolar ribonucleic acid (snoRNA) and the hsc70 messenger RNA (mRNA) in different phases of the cell cycle under normal growth conditions. RNA samples were prepared from cells in the M (lane 1), early G₁ (lane 2), G₁ (lane 3), S (lane 4), late S (lane5), and G₂ (lane 6) phases of the cell cycle, respectively. Five micrograms of the total RNA were separated on a 1.2 % agarose gel containing formaldehyde and blotted onto nitrocellulose, and hybridized with the hsc70 probe. For U14 analysis, equal amounts of total RNA were separated on a 10 % polyacrylamide gel, electro-blotted onto a GeneScreen filter, and hybridized with a U14 probe made from intron 5 of the hamster hsc70/U14 gene (Chen et al 1996a). Hybridization conditions are described under “Materials and Methods”. The figure represents an autoradiogram obtained after a 48-hour exposure