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. 2016 Nov 4;6(11):e492. doi: 10.1038/bcj.2016.93

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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USP14 and UCHL5 expression in primary WM cells, WM cell lines and bone marrow aspirate from WM patients. USP14/UCHL5 mRNA expression was examined in PBMCs from healthy donors (donors 1 and 2), primary WM cells (WM1 and WM2) the BCWM.1 cell line and its IR BCWM.1/IR derivative by real-time quantitative PCR (qPCR). POLR2A was used as a reference gene and fold change was calculated relative to mRNA expression in donors. (a) USP14 mRNA was increased in all WM cells tested with highest expression observed in WM2 patient cells (19-fold increase). (b) Similarly, UCHL5 mRNA was assessed and demonstrated more variability, with highest expression (~3-fold increase) in WM2 patient cells. (c) Immunohistochemistry (IHC) analysis was conducted on bone marrow aspirate from WM patients (total n=6, WM6 and WM3 shown as representatives) and stained for USP14 and UCHL5. Expression, distribution and staining intensity were noted to be greater in bone marrow of WM patients compared with that of healthy donors (N5 and N6). Inset IHC images are shown at × 40 magnification.