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. 2016 Dec 9;11(12):e0167937. doi: 10.1371/journal.pone.0167937

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© 2016 Shabnam et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Potential of isolated spinach thylakoids/chloroplasts to evolve O₂ rapidly on exposure to light of 600 μmol photons m^-2s^-1. Rate of photosystem (PS) II dependent O₂ evolution was measured polarographically according to Shabnam et al. [8], using Oxygraph system enabled Clark type liquid phase O₂ electrode (Hansatech Ltd., UK). Thylakoids/Chloroplasts equivalent to 20 μg of chlorophyll were used for each assay. It is evident that isolated thylakoids/chloroplasts evolve O₂ only in presence of light.