Fig 4. Statin treatment in combination with novel or accustomed exercise increases serum amyloid A content and atrogin-1 gene expression in skeletal muscle, but does not increase fiber damage or impact fiber repair.

(A) Serum amyloid A content and (B) atrogin-1 mRNA, (C) IgG⁺ myofibers, and (D) CLN⁺ myofibers in gastrocnemius muscles after normal cage activity (Sedentary), two weeks of wheel running in combination with saline or simvastatin (20 mg/kg) (Novel), or two weeks of wheel running in combination with saline or simvastatin after two weeks of exercise training (Accustomed). Representative images of IgG⁺ and CLN⁺ fibers in muscle are presented for (C) and (D). Means ± SD; n = 8-10/group. ^§ = statin x exercise interaction, p<0.05; † = main effect of statin, p<0.05; * = within group pairwise comparison, p<0.05.