Fig. 2.

 Putative binding sites between actin and sHsps. This figure shows the multiple alignment of 2 regions of actin involved in actin-actin contacts and a region relatively well conserved between the 8 sHsps (Krief et al 1999; Benndorf et al 2001) and their corresponding position in the molecule. The actin residues G63 and I64 located in subdomain 2, and P243, D244, G245 located in subdomain 4, indicated in bold, are involved in actin-actin contacts (Holmes et al 1990). L65 to K68 form a β-sheet (Kabsch et al 1990). In Hsp27, T162 to A167 is also predicted to form a β-sheet (de Jong et al 1998; Kim et al 1998)