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. 2016 Dec 9;11(12):e0168266. doi: 10.1371/journal.pone.0168266

Fig 1. Shh activation and FN production accompany cholangiocyte differentiation.

Fig 1

A. Stepwise differentiation toward cholangiocytes in five phases: induced pluripotent stem cells (iPSC), definitive endoderm (DE), hepatic specification (HS), hepatic progenitor (HP), and iPSC-derived cholangiocytes (iDC). B. RNA sequencing data showing upregulation of Shh, FN, and the biliary cytokeratins, CK7 and CK19. C. RT-PCR showing increases in Shh, FN, CK7, and CK19 mRNA levels. The data are shown as means ± standard error. *p < 0.05.