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. 2016 Dec 9;11(12):e0168266. doi: 10.1371/journal.pone.0168266

Fig 3. Gli1 binding activates transcription of FN, CK7, and CK19.

Fig 3

A. RT-PCR for Gli1 shows increased mRNA during cholangiocyte differentiation. B. Western blotting demonstrates increased Gli1 protein during cholangiocyte differentiation. Bar graphs on the right depict the densitometry results. C. ChIP assay revealing increased Gli1 binding to the FN, CK7, and CK19 promoters with increasing Shh dosing FN (++Shh = 100 ng/ml Shh administered for 8 days, +Shh = 100 ng/ml Shh administered for 4 days, -Shh = no Shh administration). D. Luciferase assays demonstrating basal and Shh-stimulated luciferase activity with the FN promoter before and after truncation. E. Luciferase assays demonstrating basal and Shh-stimulated luciferase activity with the CK7 promoter before and after truncation. F. Luciferase assays demonstrating basal and Shh-stimulated luciferase activity with the CK19 promoter before and after truncation. The data are shown as means ± standard error. *p < 0.05.