Figure 1.

Brain-specific deletion of ADK in Adk^Δbrain mice. A, Transgenic strategy: exon 7 of the Adk allele is flanked with loxP sites in Adk^fl/fl mice. B, Adk PCR on genomic DNA extracts from the cortex (C), striatum (S), hippocampus (Hp), cerebellum (Cb), heart (Ht), lung (L), liver (Lv), and spleen (Sp) from Adk^fl/fl and Adk^Δbrain mice. Tail DNA from an Adk^fl/fl (T) and wild-type mouse (WT) were included as positive controls. Water (−) was included as a no-template control. Adk forward and reverse primer (P1 and P2) sites are depicted in A. C, ADK (40 kDa) Western blots on cortical, striatal, and hippocampal protein extracts from Adk^fl/fl and Adk^Δbrain mice; n = 2/genotype are used as representatives. ADK Western blots were reprobed with GAPDH (37 kDa) as a loading control. D, E, ADK immunohistochemistry of cortical brain tissue from Adk^fl/fl (D) and Adk^Δbrain (E) mice. F, G, Nissl stain of hippocampal formation from Adk^fl/fl (F) and Adk^Δbrain (G) mice.