Figure 4.

The recurved bristle-mediated defensive behavior exhibits spatial precision. Fruit flies used mid-legs or hind legs, respectively, in responding to mechanical stimuli on the proximal (A) or distal (B) part of wing margin (n = 9–13). The Nan-Gal4 and UAS-TNT datasets (both flies exhibited normal behaviors) were grouped for statistical analysis to assess the difference between behavioral patterns (mid-leg or hind leg) when different wing region (A or B) was touched. Two-way ANOVA followed by Tukey's test was used for comparison; ****p < 0.0001. Error bars indicate SEM. Nan-Gal4, TNT: Nan-Gal4/UAS-TNT.