Figure 5.

Impaired NPC migration by Tat-containing conditioned media and recombinant Tat protein. Neurospheres derived from mouse NPCs at the seventh day of cultures were trypsinized to obtain single cells. A, B, The single cells were cultured in the presence of conditioned media from Tat-transfected and C3-transfected human primary astrocytes (A) or recombinant Tat protein (B) for 48 h, followed by quantitation of the migration distance from the center of 20 randomly selected neurospheres. The data were mean ± SD of triplicates in each group and representative of three independent experiments.