Figure 6.

Impaired NPC differentiation by recombinant Tat protein. Neurospheres derived from mouse NPCs at the seventh day of cultures were trypsinized to obtain single cells. A, B, Those single cells were cultured in the presence of 0, 2, and 5 ng/ml recombinant Tat protein for 2, 4, and 8 d and harvested for Western blotting (A) or stained for MAP-2, GFAP antibodies, followed by goat anti-mouse Alexa fluor 555 and goat anti-rabbit Alexa fluor 488 and DAPI (B; only for neurospheres at day 8). The blots/images were representative of all the stained neurospheres in each group and were representative of three independent experiments.