Figure 7.

Rescue of normal NeuN, DCX, and GFAP expression in neurospheres by Tat immunodepletion and heat inactivation. Neurospheres derived from mouse NPCs at the seventh day of cultures were trypsinized to obtain single cells. A, B, The single cells were cultured in the presence of conditioned media and Tat-immunodepleted condition media from Tat-transfected or C3-transfected human primary astrocytes for 8 d and harvested for Western blotting (A), or in the presence of 5 ng/ml recombinant Tat or heat-denatured recombinant Tat (Tat^#) for 8 d and harvested for Western blotting (B). Isotype-matched IgG was used as an immunodepletion control (A). PBS was included as a solvent control (B). The data were representative of three independent experiments.