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Two-dimensional Western blot analysis of EGFP expressed in L929 cells. EGFP constitutively expressed in stably transfected L929 cells exhibited a single isoform in nonheated cells (a) and immediately after heat shock (b: 1 hour, 43.0°C). A polyclonal antibody to EGFP was used to detect the protein, and the arrows indicate another protein that was also detected by this antibody
