Fig. 1.

 Proliferative responses of splenocytes to various stimuli. The concentrations of the different stimuli were: ConA and LPS at 2 μg/mL; MBP, MBP-Hsp70, MBP-Nt70, and MBP-Hsp83 at 12 μg/mL. Polymyxin B at 2 μg/mL was added to the wells containing the MBP-Hsp83 stimulus. The different stimuli were untreated, denatured at 100°C, or treated with trypsin before being added to the splenocyte cultures. Splenocytes were incubated for 72 hours at 37°C in 5% CO₂. Values represent the mean counts per minute and standard deviations of triplicate samples and are representative of 3 experiments. ConA, concanavalin A; LPS, lipopolysaccharide; MBP, maltose-binding protein; Hsp, heat shock protein; Nt, N-terminal domain