Fig. 3.

 Determination of B cell and T cell populations in splenocyte cultures after stimulation with the different stimuli. After incubation during the indicated days with ConA (A), LPS (B), none (C), MBP-Hsp70 (D), MBP-Nt70 (E), and MBP-Hsp83 (F), cells were labeled with either anti-CD3 (T cells) or anti-B220 (B cells) and were analyzed by flow cytometry to determine the percentage of T and B cells. The numbers of B and T cells in the splenocyte cultures were obtained by multiplying the percentages of CD3⁺ and B220⁺, respectively, and the total number of viable cells as determined by trypan blue staining. ConA, concanavalin A; LPS, lipopolysaccharide; MBP, maltose-binding protein; Hsp, heat shock protein; Nt, N-terminal domain