Fig. 5.
Proliferation of purified B cells in the presence of Leishmania Hsps. B cells, purified by negative selection from BALB/c mice spleen, were cultured at 2 × 105 cells per well and stimulated with ConA (2 μg/mL), LPS (2 μg/mL), MBP (12 μg/mL), MBP-Hsp70 (12 μg/mL), MBP-Nt70 (12 μg/mL), and MBP-Hsp83 (12 μg/mL) for 72 hours. [3H]-Thymidine was added for the last 24 hours of culture. Results are presented as the stimulatory index and standard deviations of triplicate cultures of B cells stimulated with the different stimuli