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. 2016 Dec 9;11(12):e0167763. doi: 10.1371/journal.pone.0167763

Fig 4. SrtE1 exhibits specificity for alanine at site P3 in the sorting signal.

Fig 4

Hydrolysis activity of SrtE1 or SaSrtA towards LPETG or LAETG peptide substrates was determined with an established in vitro HPLC assay [48]. The enzyme cleaves the peptide at the threonine-glycine scissile bond, producing N- and C-terminal peptide products. The extent of hydrolysis was measured by integrating the area of the N-terminal peptide product peak in the HPLC chromatogram. Error bars indicate the standard deviation of integrated HPLC peak area obtained from duplicate hydrolysis reactions.