Figure 3.

CRISPR-Cas9 gene deletion can be enhanced with CD47-Lenti for SIRPA expressing cells. A549 cells were transduced with either control or CD47-Lenti encoding a CRISPR Cas9 with guide strand targeting GFP in an already integrated GFP-lamin-A. GFP expression was assessed by flow cytometry (left), and both vectors resulted in >100-fold decrease in Lamin-A levels from a small %-age low/negative cells for GFP-lamin-A. The CD47-Lenti was able to deliver to 50% more cells than the control Lenti.