Table 2.
Strain | Genotype | Hypothetical function | IAA production in CNPS [nM] | IAA production in CNPS + trp [nM] |
---|---|---|---|---|
DSM 17395 |
Wild type | 0.4 | 10.8 | |
3756 | PGA1_c11890:: Tn5 (KanR) | Indoleacetamide hydrolase | Yes | Yes |
3796 | PGA1_c11890:: Tn5 (KanR) | Indoleacetamide hydrolase | Yes | Yes |
1630 | PGA1_c16910:: Tn5 (KanR) | Indole-3-glycerol phosphate synthase | NA* | Yes |
1397 | PGA1_c23870:: Tn5 (KanR) | Nitrile hydratase subunit beta | Yes | Yes |
3422 | PGA1_c31390:: Tn5 (KanR) | Aromatic-L-amino-acid decarboxylase | Yes | Yes |
*NA - Not available since this mutant does not grow in these conditions without the addition of tryptophan.