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. 2016 Dec 1;5:e18351. doi: 10.7554/eLife.18351

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© 2016, Shin et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 7. — (A) Map of PAX3 phosphorylation sites determined by mass spectrometry. Sites shown in green have been published before (phosphosite.org) and sites displayed in black were newly identified. The red asterisk marks a phosphorylation, which was strongly induced by co-transfection with CA Braf (V600E). (B) Scatter plot showing the distribution of SILAC log2 phosphosite ratios normalized to PAX3 protein expression levels after transfection with Pax3 and Braf (heavy) and Pax3 (light). (C) Histogram of log2 PAX3 phosphosite ratios in two independent experiments (exp1: light grey; exp2: black) normalized to PAX3 protein expression ratios. (D) Representative MS SILAC spectra for identification of S222. Andromeda Score for corresponding MS/MS spectra is 236.38. The mass deviation is given in p.p.m.

DOI: http://dx.doi.org/10.7554/eLife.18351.012

Figure 7—source data 1. Source data for mass spectrometry analysis.
DOI: http://dx.doi.org/10.7554/eLife.18351.013

elife-18351-fig7-data1.xlsx^{(4.2MB, xlsx)}

DOI: 10.7554/eLife.18351.013