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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Small. 2016 Aug 16;12(38):5256–5260. doi: 10.1002/smll.201601540

Figure 1.

Figure 1

GPCR incorporation into diblock copolymer bilayer vesicles, pGUPs. (A) Schematic of pGUP formation and protein incorporation. Films of protein, agarose, and polymer are made on a coverslip and rehydrated with a sucrose buffer solution containing BODIPY-GTPγS. pGUPs formed of diblock copolymer bilayers can be lyophilized and the GPCR retains its function (steps 2–4); for enlarged image see Supporting Information (Figure S1). (B) Confocal micrographs of pGUPs prior to lyophilization. The left micrograph shows the polymer bilayer tagged with ATTO-488-DPPE. The right micrograph shows that rhodamine antibody-tagged 5-HT1AR is evenly distributed throughout the polymer bilayer. (C) The left image shows a pGUP sample after lyophilization. Upon rehydration, pGUPs can still be detected as shown in the right micrograph. All scale bars represent 5 µm.