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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Allergy Clin Immunol. 2016 Aug 13;138(6):1741–1744.e9. doi: 10.1016/j.jaci.2016.06.048

Figure 1. Basophil activation and association with platelets after peanut stimulation.

Figure 1

(A) Expression of each marker by basophils stimulated with peanut (Δ median intensity (MI) vs. media control). Expression at 15min is shown for all markers except FcεRI, CD32 and CD38 where 30min is shown. (B) Expression of CD63 and CD61 (MI) by basophils after 15min stimulation. *p < 0.05 vs media. (C) Representative plot of CD61 expression (%) by basophils from peanut-allergic subjects. (D, E) Expression of CD63 and pp38 (%) by free basophils (CD61-) or basophils+platelets (CD61+) from peanut-allergic patients. *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. free basophils. (F) Imaging flow cytometry of basophils-platelet complexes from a healthy control stimulated with anti-IgE for 30min. Two representative images of activated basophils are shown. BF, bright field. (G) Expression of pERK (15min), pp38 (15min) and pS6 (30min) by basophils. *p < 0.05, **p < 0.01.