Skip to main content
. Author manuscript; available in PMC: 2018 Jan 28.
Published in final edited form as: Cancer Lett. 2016 Oct 22;385:207–214. doi: 10.1016/j.canlet.2016.10.021

Figure 3. SPOP, the adaptor protein of Cullin3 family E3 ligase, specifically interacts with Cdc20 through its N-terminal MATH domain.

Figure 3

A) Immunoblot (IB) analysis of whole cell lysates (WCL) and immunoprecipitates (IP) derived from 293T cells transfected with indicated constructs. 36 hours post-transfection, cells were pretreated with 10 µM MG132 for 10 hours before harvesting.

B) IB analysis of GST pull-down precipitates from 293T cell lysate with overexpression of HA-Cdc20 using bacterially purified GST and GST-SPOP proteins.

C) Schematic of SPOP with MATH and BTB domain.

D) IB analysis of WCL and anti-Flag IP derived from 293T cells transfected with indicated constructs. 36 hours post-transfection, cells were pretreated with 10 µM MG132 for 10 hours before harvesting.