Fig 7.

Association of purified tubulin and p26. Purified Artemia tubulin at 1.2 μg/μL was incubated at 35°C for 7 hours in the presence (A, B) or absence (C) of p26 in Pipes buffer. Solutions were centrifuged at 200 000 × g for 3 (A, B) or 22 hours (C) in 17–40% continuous sucrose gradients and fractionated, and samples from each fraction were electrophoresed in sodium dodecyl sulfate polyacylamide gels before blotting to nitrocellulose and immunostaining. The membranes were probed with DM 1A (A), anti-p26 (B), and α/β-tubulin (C). The top of each gradient is to the left