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. 2017 May 1;81(9):737–747. doi: 10.1016/j.biopsych.2016.06.005

Figure 3.

Figure 3.

5-Hydroxytryptamine 2C receptors (5-HT2CRs) in dopamine (DA) neurons mediate effects of 5-HT drugs to inhibit binge-like eating. (A) Illustration of 5-HT2CR expression pattern in wild-type (WT), 2C-null, DA-2C-RE or DA-2C-KO mice. Blue indicates the distribution of 5-HT2CRs (note that 5-HT2CRs are reported to be exclusively in the brain); the red box indicates DA neural population. (B) Effects of intraperitoneal (i.p.) injections of saline, fluoxetine (10 mg/kg), or d-fenfluramine (d-Fen) (3 mg/kg) on binge-like eating (2.5-hour high-fat diet [HFD] intake) in four groups of mice that all received tamoxifen and were exposed to intermittent HFD feeding. n = 6 to 9 per group. Results are shown as mean ± SEM. *p < .05 between drug treatment and saline treatment in the same genotype in two-way analyses of variance (ANOVAs) followed by post hoc Sidak tests. (C) Effects of i.p. injections of saline, lorcaserin (3 mg/kg), or lorcaserin (6 mg/kg) on 2.5-hour HFD intake in WT mice exposed to intermittent HFD feeding or continuous chow/HFD feeding. n = 10 to 12 per group. Results are shown as mean ± SEM. *p < .05 between drug treatment and saline treatment in the same feeding paradigm in two-way ANOVAs followed by post hoc Sidak tests. (D) Effects of i.p. injections of saline or lorcaserin (6 mg/kg) on binge-like eating (2.5-hour HFD intake) in four groups of mice that all received tamoxifen and were exposed to intermittent HFD feeding. n = 7 to 10 per group. Results are shown as mean ± SEM. *p < .05 and **p < .01 between lorcaserin treatment and saline treatment in the same genotype in two-way ANOVAs followed by post hoc Sidak tests. (E) Effects of i.p. injections of saline or lorcaserin (6 mg/kg) on 2-hour refeeding after an overnight fast in four groups of mice that all received tamoxifen and were maintained on chow diet. n = 7 to 15 per group. Results are shown as mean ± SEM. *p < .05 between lorcaserin treatment and saline treatment in the same genotype in two-way ANOVAs followed by post hoc Sidak tests. KO, knockout; ns, not significant; RE, re-expression.