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. Author manuscript; available in PMC: 2017 Jun 24.
Published in final edited form as: Science. 2016 Jun 24;352(6293):1535–1542. doi: 10.1126/science.aaf7419

Fig. 4. TLR-ligands are endocytosed by a sentinel GC.

Fig. 4

(A-D) Colonic explants were treated as indicated, whole mounted and visualized by confocal microscopy: (A-C) x/y-axis cross-sections (upper), x/z-axis cross-sections (yellow boxes), DNA (blue), actin (grey). (A) RedMUC298trTg tissue: magnified x/z-axis cross-section regions indicated by white boxes (lower), LPS/P3CSK4 (green), mCherry-MUC2 (red). (B) WT with or without Casp. IP, Tlr2−/− , and MyD88−/− tissue: P3CSK4 (green). (C) RedMUC298trTg tissue: endocytotic GC (yellow arrow), non-endocytotic GC (white arrow), mCherry-MUC2 (red), dextran (green). (D) P3CSK4/dextran co-treated WT colon. (E) Quantification of endocytotic cells. Errors SEM of 3-4 animals; significance by Tukey’s multiple comparison (* p<0.05). Scales 20 μm.