Fig. 10.

Effect of PGE2, butyrate, NF-κB–selective inhibitor (BAY11-7082), or COX2-selective inhibitor (SC 58635) on lipolysis in TNF-α–stimulated 3T3-L1 cells after 24 h. Concentrations of FFAs (a) and free glycerol (b) in the co-culture medium were determined using an assay kit. As the control, the concentrations of FFAs and free glycerol were determined in the medium of untreated and non–co-cultured 3T3-L1 cells. Values from five independent experiments are expressed as the mean ± SD. *p < 0.05 and ***p < 0.001 versus TNF-α–stimulated 3T3-L1 cells, as determined by ANOVA and the Tukey-Kramer test; ^† p <0.05, ^††† p <0.001 for TNF-α (+) and PGE2 (−) and SC 58635 (+) versus TNF-α (+) and PGE2 (+) and SC 58635 (+), as determined by paired t-test