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. 2016 Dec 9;15:213. doi: 10.1186/s12944-016-0387-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of pertussis toxin (PTX) on the production of PGE2 induced by 24 h of co-culture using the contact method. The concentration of PGE2 in the co-culture medium was determined by ELISA. The concentration of PGE2 in the medium of non–co-cultured 3T3-L1 or RAW 264.7 cells served as the control. Values from five independent experiments are expressed as the mean ± SD. ***p < 0.001 versus co-culture, and ^### p < 0.001 versus co-culture with PTX, as determined by ANOVA and the Tukey-Kramer test