Fig. 5.

Effects of butyrate on AdPLA protein (a, b) and mRNA (c) expression in 3T3-L1 cells co-cultured using the transwell method for 24 h. AdPLA and actin proteins were detected by western blotting (a). The expression ratio of AdPLA to actin was determined based on quantification of the relative density of western blotting bands (b). Ala2g16 and β-actin mRNAs were detected using qRT-PCR (C), as described in the Methods. For western blotting and protein expression, n = 3, and for qRT-PCR, n = 5. Values are expressed as the mean ± SD. *p < 0.05, and **p < 0.01, ***p < 0.001 versus the control, as determined by ANOVA and the Tukey-Kramer test