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. 2016 Dec 9;15:214. doi: 10.1186/s12944-016-0383-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Representative images of 3T3-L1 cells on D0 and D12 during the differentiated process and Oil red O staining of differentiated 3T3-L1 cells on D12. b TG content assay of 3T3-L1 cells on different day in the differentiated process. Intracellular TG contents were normalized by total protein. c 3T3-L1 cells were induced to differentiated adipocytes according to the protocol. Proteins in the cells of different days on the differentiated process were separated by SDS-PAGE and immunoblotted for PPARγand β-actin. Representative Western blot results are shown. The data were presented as the mean ± SD. * p <0.05, **p <0.01 versus the group of D0