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. 2016 Summer;15(3):471–481.

Table 5.

Determination of antimutagenic potency of A. ciniformis oil by S. typhimurium strains TA100 and TA98 with S9.

Test Negative Control Positive Control TA100/TA98
0.1 mL 0.1 mL 0.1 mL Test strain
(approximately 1/6×106 cells/ml)
200 µL - 200 µL 2-aminoanthracene (1µg/µl in DMSO)
10 µL - - Test concentration (oil)
0.5 mL 0.5 mL 0.5 mL S9 mix
2 mL 2 mL 2mL Top agar