Figure 2.

Effects of resveratrol (RES) on β-catenin, MnSOD, and PPAR-δ gene expression by real-time PCR. PBMCs were treated with 50 µM RES for 12 h incubation. a) A non-significant change in MnSOD mRNA was observed in healthy subjects after treatment with RES as compared to blank. Treatment with RES could significantly increase the mRNA level of MnSOD in CAD patients compared to blank. b) No significant differences were observed for β-catenin mRNA in healthy subjects and CAD patients after treatment with RES compared to their blank. c) No significant differences in PPAR-δ mRNA were observed for both healthy subjects and CAD patients after treatment with RES compared to their blanks. d) Between-group analysis showed non-significant higher levels of MnSOD, β-catenin and PPAR-δ in healthy subjects than CAD patients in both blank and RES treatment. RES was dissolved in DMSO, and blank groups (untreated cells) were containing only DMSO. In both blank and RES treated cells, DMSO was present at equal concentration (0.025%). All gene expression tests were performed in triplicate in each experiment, and the mean of duplicates were used for statistical analyses. Data are expressed as means ± SEM.