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Reaction progress curves for the conversion of 5 to 8 at pH 7.4 and 37 °C (220 nm). A. [5]0 = 1000 μM. B. [5]0 = 500 μM. C. [5]0 = 250 μM. D. [5]0 = 125 μM. E. [5]0 = 62.5 μM. F. [5]0 = 31.3 μM.