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Reaction progress curves for the hydrolysis of 5 ([5]0 = 250 μM) at variable temperature and pH 7.4 (220 nm). A. 9 °C. B. 18 °C. C. 28.5 °C. D. 37 °C. E. 45 °C. F. 55 °C. G. 60 °C. H. 65 °C. I. 70 °C. J. 75 °C.