Table 1.

Initial rates of hydrolysis of 5 and observed formation of 8 (pH 7.4, 37 °C) by Sinapis alba myrosinase. The concentration of myrosinase was constant (8.83 U ml⁻¹).

[Gluc]0(μM)	Δ[5] Δt−1(μM min−1)
	220 nm	227 nm	235 nm	241 nm
1000	−4.11	−4.24	−4.16	−4.31
500	−2.74	−2.82	−2.89	−2.77
250	−1.75	−1.70	−1.67	−1.64
125	−1.12	−1.06	−1.07	−1.03
62.5	−0.65	−0.66	−0.71	−0.70
31.3	−0.46	−0.48	−0.51	−0.48
	Δ[8]obsΔt−1(μM min−1)
1000	4.48	4.83	4.65	4.76
500	2.70	2.99	3.89	3.54
250	1.63	1.67	1.71	1.68
125	0.96	1.00	1.10	1.05
62.5	0.62	0.72	0.74	0.67
31.3	0.45	0.46	0.49	0.47