Table 2.
Enzyme-dependence on the rate of conversion for 5 ([5]0 = 250 μM) to 8 (pH 7.4, 37 °C). The concentration of myrosinase ([Myr]) was 100%, 67%, 33%, and 0% of a maximum value (8.83 U ml−1). Reaction progress curves for [ITC]t with 0% [Myr] were not generated due to negligible levels of detected [ITC] [1].
| [Myr] (relative) |
Δ[5] Δt−1(μM min−1) |
|||
|---|---|---|---|---|
| 220 nm | 227 nm | 235 nm | 241 nm | |
| 100% | −1.75 | −1.70 | −1.67 | −1.64 |
| 67% | −0.95 | −0.93 | −0.91 | −1.01 |
| 33% | −0.58 | −0.57 | −0.53 | −0.48 |
| 0% | 0.03 | 0.06 | 0.05 | 0.00 |
| Δ[8]obsΔt−1(μM min−1) | ||||
| 100% | 1.63 | 1.67 | 1.71 | 1.68 |
| 67% | 0.96 | 0.89 | 1.00 | 0.96 |
| 33% | 0.47 | 0.46 | 0.45 | 0.46 |