Table 4.
pH Dependence of the action of Sinapis alba myrosinase on the rate constant for observed ITC formation ([Gluc]0 = 250 μM, 37 °C).
pH |
Δ[8]obsΔt−1[Myr]−1(min−1) |
Δ[9]obsΔt−1[Myr]−1(min−1) |
|||||
---|---|---|---|---|---|---|---|
220 nm | 227 nm | 235 nm | 241 nm | 227 nm | 235 nm | 241 nm | |
2.0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
3.0 | 0.08 | 0.09 | 0.09 | 0.08 | 0.40 | 0.41 | 0.39 |
4.0 | 0.32 | 0.31 | 0.33 | 0.33 | 1.07 | 1.09 | 1.09 |
5.0 | 0.34 | 0.34 | 0.35 | 0.35 | 1.29 | 1.31 | 1.31 |
6.0 | 0.31 | 0.31 | 0.33 | 0.32 | 1.15 | 1.16 | 1.16 |
7.4 | 0.18 | 0.19 | 0.19 | 0.19 | 1.23 | 1.28 | 1.27 |
8.0 | 0.20 | 0.18 | 0.19 | 0.18 | 1.31 | 1.37 | 1.27 |
9.0 | 0.17 | 0.16 | 0.17 | 0.17 | 1.08 | 1.11 | 1.10 |
10.0 | 0.16 | 0.15 | 0.18 | 0.16 | 0.98 | 1.03 | 1.03 |